The game between Laois and Carlow was initially postponed from Wednesday evening to the weekend due to weather conditions.
The latest O'Byrne Cup cancellation leaves Laois without an opponent for their last game in the competition.
Carlow were due to play Billy Sheehan's footballers in Stradbally on Wednesday until the game was postponed due to a waterlogged pitch.
Today Leinster GAA were notified that Carlow were unable to fulfil the fixture due to "Sigerson Cup commitments, injuries and sickness ".
The game will now, not take place.
Laois' first game of their division four league campaign will them making the trip to Markievicz Park to take on Sligo.
Full Interview: Portarlington Footballer Ronan Coffey
Full Interview: The Down's Manager Dermot Power
St Brigid's And St Joseph's Aiming To Reach Leinster Semi-Finals
Ballinagar And Milltown To Meet In Leinster Intermediate Quarter-Final
Ronan Coffey Praises Portarlington’s Young Guns Ahead Of Leinster Quarter-Final
Mauricio Pochettino Set To Be Appointed Manchester United Manager
Darragh Murray Named In Irish Squad For Fiji Clash
Portlaoise Panthers Aiming To Bounce Back In Division One
Portarlington On The Road In Leinster
The Down's Building Towards Leinster Football Quarter-Final
Laois Provisional League Fixtures Revealed
The Harps Manager Praises His Sides Efforts In Leinster
Buccaneers Claim Back To Back AIL Wins
Kilcormac Killoughey Captain Full Of Praise For Goalkeeper
Lough Lene Gaels Suffer Late Heartbreak In Leinster Quarter-Final
Crookedwood Win Leinster Junior Camogie Title
Conor Slevin Wins Dramatic Leinster Quarter-Final For Kilcormac Killoughey
Ireland Secure Comfortable Win Over Argentina
Kilcormac Killoughey Seal Win But Heartbreak For Lough Lene Gaels And The Harps