The game between Laois and Carlow was initially postponed from Wednesday evening to the weekend due to weather conditions.
The latest O'Byrne Cup cancellation leaves Laois without an opponent for their last game in the competition.
Carlow were due to play Billy Sheehan's footballers in Stradbally on Wednesday until the game was postponed due to a waterlogged pitch.
Today Leinster GAA were notified that Carlow were unable to fulfil the fixture due to "Sigerson Cup commitments, injuries and sickness ".
The game will now, not take place.
Laois' first game of their division four league campaign will them making the trip to Markievicz Park to take on Sligo.