The incident has largely affected brown trout the waterway in Tullamore town.
A fish kill is being investigated in an Offaly river which has lead to the death of just under 70 fish.
The mass incident has mainly affected brown trout along a kilometre of the water body in Tullamore town.
Personnel from Inland Fisheries Ireland are leading the case, and working with the local authority and the Environmental Protection Agency to investigate the fish kill,
The IFI is advising members of the public not to consume any dead fish found at waterways in the area.
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